\\How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- Why didn't the oligos nanodrop??
- Why didn't the primers bind to LS_miR_144?
miRNA Sensors (Lab Work)
- Golden Gate
- Low Sensor miR-144-miR-181c
- Transform
- High Sensor miR-30d
- High Sensor miR-146a
- High Sensor let-7f
- High Sensor miR-125b
- Low Sensor miR-144
- Low Sensor miR-181c
- Pick colonies to miniprep and midiprep
- pEXPR: MAV1212-TRE-L7ae (LR)
- pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- Prepare and run a gel (Hyperladder 2 and 4% agaros gel w/ annealed target sites)
- High Sensor miR-30d TS
- High Sensor miR-146a TS
- High Sensor let-7f TS
- High Sensor miR-125b TS
- Low Sensor miR-144 TS
- Low Sensor miR-181c TS
- FACS cell prep
- Transfect L7ae Experiment
- Analyze FACS data
NOTES
We have a new primer to sequence with (again, from Jeremy)
We now have FACS DATA
Single Stranded Annealed
Let-7f | 125b | 144 | 181c | 30d | 146a | Hyperladder 2 | Let-7f | 125b | 144 | 181c | 30d | 146a
