How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- What's going on with the double input low sensor
- What's the deal with MAV1212: hEF1a-GAL4VP16 (midi???)
miRNA Sensors (Lab Work)
- Digest and Run Gel
- pEXPR: MAV1212-hEF1a-TAL14
- Inoculate midi prep culture (if digest works)
- pEXPR: MAV1212-hEF1a-TAL14
- LR
- pEXPR: MAV1212-TRE-L7ae (again since the digest failed repeatedly??)
- Pick colonies (even from sensor plates)
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Make new Amp/Xgal plates
- Transform (give to treatment group)
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- pEXPR: MAV1212-hEF1a-GAL4VP16
- pEXPR: MAV1212-TRE-GAL4VP16
- Split & Seed for L7ae experiment take 3
- Dilute MAV1212-TAL14-mKate to 500 ng/uL and move to TC room
NOTES
Hyperladder 1kb | MAV1212-hEF1a-TAL14 (digested) | MAV1212-hEF1a-TAL14 (undigested) | unloaded
