Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Meet with Kyle about quantifying repression
- Troubleshoot gel results
miRNA Sensors (Lab Work)
- Digest and run gel
- MAV1212-hEF1a-TAL14
- MAV1212-hEF1a-TAL21
- LR (if digest fails)
- MAV1212-hEF1a-TAL21 - find pENTR TAL21
- Pick colonies (if digest failes)
- pENTR TAL14
- double input low sensor