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  • The eBFP2 digest with BsrGI was a control to test whether BsrGI was working properly. If BsrGI were working properly we would have expected 3 bands. Yet there is no DNA of any length visible in this lane. I'm not sure of the reason for this but in any case this lane is useless in telling us whether or not BsrGI works. On Monday we will run a gel of non-digested GG product (eYFP-BACE1) to compare the gel pattern of undigested GG product vs digested GG product.
  • The digests with PstI were a second attempt to validate proper GG assembly of eYFP-BACE1 (because our first attempt at restriction digesting with BsrGI was a dud). Yet oddly, we again see that no digestion occurred.

AL - 7/1

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LR Bace1 and bace2, creating 5 fm concentration using the excel sheet on the protocol page.

 

ran gel of undigested gg donr and products.