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Test using the whole detection circuit and Beta Amyloid to measure circuits response to input |
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This will determine the ideal concentration of Syk-TEVp for the best signal/noise ratio as well as characterize our circuit for use in other circuits. |
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BCR (CD79A+B, IgM heavy, IgM light), Lyn, Syk-TEVp, (reporter circuit), (whatever is needed to make dox work). We will be testing all combinations of CD79(A/B)-(stop/TCS-Gal4Vp16). Dox, Beta Amyloid |
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All combinations of plasmids being tested will be prepared and put into dox ladders |
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The Expression of the reporter should be much higher in Beta Amyloid than in the previous Beta amyloid- test. |
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