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BCR (CD79A+B (with and without TCS-Gal4VP16), IgM heavy, IgM light), Lyn, Syk-TEVp, tre-mKare(dox reporter circuit), UAS-eYFP(reporter circuit), hef1a:eBFP(transfection marker), (whatever is needed to make dox work). We will be testing all combinations of CD79(A/B)-(stop/TCS-Gal4Vp16). Dox |
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We should identify what level of Syk-TEVp causes constitutive expression of the reporter and characterize the off behavior of our circuit so we will be able to compare it to the on behavior later. Our data will produce a scatter plot of eYFP (reporter) as a function of mKate (driven by the same promoter as syk). |
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