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Test using the whole detection circuit and Beta Amyloid to measure circuits response to input. This test will vary several circuit parameters to find the ideal sensor. |
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This will determine the ideal concentration what amounts of Syk-TEVp and ideal for each placement of TCS-Gal4VP16 for the best signal/noise ratio as well as characterize our circuit for use in other circuitscause excessive constitutive production of reporter so we don't need to test those for the active test. This will also characterize our circuits off behavior so we can compare it to its on behavior later to get the on/off ratio. |
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BCR (CD79A+B (with and without TCS-Gal4VP16), IgM heavy, IgM light), Lyn, Syk-TEVp, tre-mKare(dox reporter circuit), UAS-eYFP(reporter circuit), hef1a:eBFP(transfection marker), (whatever is needed to make dox work). We will be testing all combinations of CD79(A/B)-(stop/TCS-Gal4Vp16). Dox |
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