| Date | Product | Starting Plasmid | Thermocycler Variables: #cycles / annealing temp / extension time | Gel Picture |
|---|---|---|---|---|
| 6/23 | Q2-BACE2-QX | pEXPR BACE2 | 30 / 60C / 30s | Gel Picture 6/23 |
| Q1-eYFP-Q2 | pEXPR TRE:eYFP | |||
| Q1-BACE2-Q2 | pEXPR BACE2 | |||
| Q2-eYFP-QX | pEXPR TRE:eYFP | |||
| 6/24 | Q2-BACE2-QX | pEXPR BACE2 | 35 / 56.2C / 30s | Gel Picture 6/24 |
| Q1-eYFP-Q2 | pEXPR TRE:eYFP | |||
| Q1-BACE2-Q2 | pEXPR BACE2 | |||
| Q2-eYFP-QX | pEXPR TRE:eYFP | |||
| 6/25 | Q1-BACE2-Q2 | pEXPR BACE2 | 35 / 50.2C / 30s
| Gel Picture 6/25 |
| Q2-BACE2-QX | pEXPR BACE2 | |||
| Q2-BACE2-QX | pEXPR BACE2 | |||
| Q1-eYFP-Q2 | pEXPR TRE:eYFP | |||
| Q2-eYFP-QX | pEXPR TRE:eYFP | |||
| 6/26 | Q2-BACE2-QX (plus betaine) | pEXPR BACE2 | 30 / 55C / 1m
| Gel Picture 6/26 |
Q2-BACE2-QX (plus DMSO) | pEXPR BACE2 | |||
Q1-BACE2-Q2 (plus betaine) | pEXPR BACE2 | |||
Q1-BACE2-Q2 (plus DMSO) | pEXPR BACE2 | |||
| 6/27 | Q2-BACE1-QX | pEXPR BACE1 | Gel Picture 6/27 | |
| Q2-eYFP-QX | pEXPR eYFP | |||
| 6/30 | Q1-eYFP-Q2 | pEXPR eYFP | Gel Picture 6/30 | |
| Q1-BACE1-Q2 | pEXPR BACE1 | |||
| 7/9 | Q2-eYFP-QX (for BACE2) | pEXPR eYFP | 35 / 58C / 1m with both DMSO and Betaine | Gel Picture 7/9 |
| Q1-eYFP-Q2 (for BACE2) | pEXPR eYFP | |||
| Q2-eYFP-QX (for BACE1) | pEXPR eYFP | |||
| Q2-BACE2-QX | pEXPR BACE2 | |||
| Q1-BACE2-QX | pEXPR BACE2 | |||
| Q1-BACE1-QX | pEXPR BACE1 | |||
| 7/10 | (miRNA generator stuff) | 35 / 58C / 1m with both DMSO and Betaine | Gel Picture 7/10 | |
| (20 PCR products in all) | ||||
| 7/10 | (miRNA generator stuff) | |||
| (see lab notebook 7/11) | ||||
| 7/11 | (miRNA generator stuff) | |||
| 30 / 61C / 30s | Gel Picture 7/11 | |||
| (12 PCR products in all) |
Ran PCRs according to protocol on NEB website: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531
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