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miRNA Detection

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Experiment #1

13

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Day 1: Seed cells

14

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Day 2: Transfect Promoter:Gene₁ (___ ng/uL - transfection marker), Promoter:Gene₂ (___ ng/uL) and Promoter:Gene₃ (___ ng/uL) into HEK cells

15

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Day 3: Add DOX (if necessary)

16

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Day 4 (time): Flow Cytometer

DNA Dilution: __uL Promoter:Gene₁; __uL Promoter:Gene₂; __uL Promoter:Gene₃
(Eg)_x: add _:_:_
1. __uL Promoter:Gene₁-lipid complex
2. __uL Promoter:Gene₂-lipid complex
3. __uL Promoter:Gene₃-lipid complex

24-WELL PLATE TRANSFECTION MAP:

//////	//////	//////	//////	//////	//////
+ve control Promoter:Gene₁	+ve control Promoter:Gene₁	-ve control dummy DNA with Lipofectamine	-ve control dummy DNA with Lipofectamine	-ve/-ve control no DNA no Lipofectamine	-ve/-ve control no DNA no Lipofectamine
///EMPTY WELL///	//////	//////	//////	//////	//////
(Eg)₁ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₂ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₃ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₄ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₅ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₆ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃

RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

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Treatment

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