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Cloak

MAV1212-hEF1a-eBPF2

21

incomplete

Day 1: Seed cells

22

incomplete

Day 2: Transfect MAV1212-hEFa-eBFP2 (___ ng/uL - transfection marker) into HEK cells

0

incomplete

Day 4 (time)3: Flow CytomeeterCytometer

DNA Dilution: __uL Promoter:Gene₁; __uL Promoter:Gene₂; __uL Promoter:Gene₃_uL MAV1212-hEF1a-eBFP2, ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP
(Eg)_x: add _:_:_
1. __uL Promoter:Gene₁-lipid complex
2. __uL Promoter:Gene₂-lipid complex
3. __uL Promoter:Gene₃-lipid complex

24-WELL PLATE TRANSFECTION MAP:

//////	//////	//////	//////	//////	//////
+ve control MAV1212-hEF1a-BPF2	+ve control MAV1212-hEF1a-eBPF2	-ve control dummy DNA with Lipofectamine	-ve control dummy DNA with Lipofectamine	-ve/-ve control no DNA no Lipofectamine	-ve/-ve control no DNA no Lipofectamine
///EMPTY WELL///	//////	//////	//////	//////	//////
(Eg)₁ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₂ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₃ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₄ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₅ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃	(Eg)₆ __uM DOX Promoter:Gene₁ Promoter:Gene₂ Promoter:Gene₃

RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

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