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miRNA Detection

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Experiment #1

GOAL: Test that promoters work in the absences of repressors

3 MAV1212-hEF1a-eBPF2 21 incomplete Day 1: Seed cells 22 incomplete Day 2: Transfect MAV1212-hEFa-eBFP2 (___ ng/uL - transfection marker) into HEK cells 0 incomplete Day 3: Flow Cytometer

 

  • DNA Dilution: ___uL MAV1212-hEF1a-eBFP2, ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP(Eg)x: add _:_:_
  • __uL Promoter:Gene1-lipid complex
  • __uL Promoter:Gene2-lipid complex
    • __uL Promoter:Gene3-lipid complex


24-WELL PLATE TRANSFECTION MAP:

+ve control
  • MAV1212-hEF1a-BPF2
+ve control
  • MAV1212-hEF1a-eBPF2
-ve control
  • dummy DNA
  • with Lipofectamine
-ve control
  • dummy DNA
  • with Lipofectamine
-ve/-ve control
  • no DNA
  • no Lipofectamine
-ve/-ve control
  • no DNA
  • no Lipofectamine
///EMPTY WELL/////////////////////////////////
////////////////////////////////////
(Eg)1
  • __uM DOX
  • Promoter:Gene1
  • Promoter:Gene2
  • Promoter:Gene3
(Eg)2
  • __uM DOX
  • Promoter:Gene1
  • Promoter:Gene2
  • Promoter:Gene3
(Eg)3
  • __uM DOX
  • Promoter:Gene1
  • Promoter:Gene2
  • Promoter:Gene3
(Eg)4
  • __uM DOX
  • Promoter:Gene1
  • Promoter:Gene2
  • Promoter:Gene3
(Eg)5
  • __uM DOX
  • Promoter:Gene1
  • Promoter:Gene2
  • Promoter:Gene3
(Eg)6
  • __uM DOX
  • Promoter:Gene1
  • Promoter:Gene2
  • Promoter:Gene3

RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

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