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Cloak

Experiment #1

GOAL: Test that promoters work in the absences of repressors

3 incomplete Day 1: Seed cells 22 incomplete Day 2: Transfect MAV1212-hEFa-eBFP2 (___ ng/uL - transfection marker) into HEK cells 0 incomplete Day 3: Flow Cytometer

 

  • DNA Dilution: ___uL MAV1212-hEF1a-eBFP2, ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP


24-WELL PLATE TRANSFECTION MAP:

-ve control
  • dummy DNA
    • with LipofectamineEMPTY WELL//////
    +ve control

    B

    • MAV1212-hEF1a-BPF2
    		+ve control
    • MAV1212-hEF1a-eBPF2
    • eBFP2

    GWT

    • Wild Type Hef1a-2xKturn-eGFP

    GM

    • Mutant Hef1a-2xKturn-eGFP
    		////////////-ve control
    • dummy Dummy DNA
    • with With Lipofectamine

    B

    • MAV1212-
    ve/
    • hEF1a-eBFP2

    GWT

    • Wild Type Hef1a-2xKturn-eGFP

    GM

    • Mutant Hef1a-2xKturn-eGFP
    ve control
    • no DNA
    • no Lipofectamine
    -ve/-ve control
    • no DNA
    • no Lipofectamine
    		////////////-ve control
    • Dummy DNA
    • With Lipofectamine
     

     


    		 ////////////-ve/-ve control
    • No DNA
    • No Lipofectamine

     


     


    		 //////////////////////////////////////////(Eg)1
    • __uM DOX
    • Promoter:Gene1
    • Promoter:Gene2
    • Promoter:Gene3
    		(Eg)2
    • __uM DOX
    • Promoter:Gene1
    • Promoter:Gene2
    • Promoter:Gene3
    		(Eg)3
    • __uM DOX
    • Promoter:Gene1
    • Promoter:Gene2
    • Promoter:Gene3
    		(Eg)4
    • __uM DOX
    • Promoter:Gene1
    • Promoter:Gene2
    • Promoter:Gene3
    		(Eg)5
    • __uM DOX
    • Promoter:Gene1
    • Promoter:Gene2
    • Promoter:Gene3
    		-ve/-ve control
    • No DNA
    • No Lipofectamine
    (Eg)6
    • __uM DOX
    • Promoter:Gene1
    • Promoter:Gene2
    • Promoter:Gene3


    RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

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