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Cloak

Experiment #1

GOAL: Test the strength of L7ae

3

incomplete

Day 1: Seed cells

22

incomplete

Day 2: Transfect MAV1212-hEF1a-eBFP2 (___ ng/uL - transfection marker), Mutant hEF1a-2xKturn-eGFP (___ ng/uL) and Wild Type hEF1a-2xKturn-eGFP (___ ng/uL) into HEK cells

0

incomplete

Day 3: Flow Cytometer

DNA Dilution: ___uL MAV1212-hEF1a-eBFP2, ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP

24-WELL PLATE TRANSFECTION MAP:

-ve control

Dummy DNA
With Lipofectamine

-ve control

Dummy DNA
With Lipofectamine

//////	//////	//////	//////	//////	//////
(B)1 MAV1212-hEF1a-eBFP2	(B)₂ MAV1212-hEF1a-eBFP2
-ve/-ve control No DNA No Lipofectamin	-ve/-ve control No DNA No Lipofectamine	(G_M)1 Mutant Hef1a-2xKturn-eGFP	(G_M)₂ Mutant Hef1a-2xKturn-eGFP	Mutant Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2	Mutant Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2	Mutant Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2 hEF1a-L7ae	Mutant Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2 hEFa-L7ae
(G_WT)1 Wild Type Hef1a-2xKturn-eGFP	(G_WT)₂ Wild Type Hef1a-2xKturn-eGFP	Wild Type Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2	Wild Type Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2	Wild Type Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2 hEFa1-L7ae	Wild Type Hef1a-2xKturn-eGFP MAV1212-hEF1a-eBFP2 hEF1a-L7ae
-ve control Dummy DNA With Lipofectamine	-ve control Dummy DNA With Lipofectamine	-ve/-ve control No DNA No Lipofectamin	-ve/-ve control No DNA No Lipofectamine

RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

Experiment #2

GOAL: Test the strength of L7ae

2

incomplete

Day 1: Seed cells

1

incomplete

Day 2: Transfect Promoter:Gene₁ (___ ng/uL - transfection marker), Promoter:Gene₂ (___ ng/uL) and Promoter:Gene₃ (___ ng/uL) into HEK cells

7

incomplete

Day 3: Add DOX (if necessary)

10

incomplete

Day 4 (time): Flow Cytometer

DNA Dilution: ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP, ___ uL hEF1a-L7ae
(Eg)_x: add _:_:_
1. __uL Promoter:Gene₁-lipid complex
2. __uL Promoter:Gene₂-lipid complex
3. __uL Promoter:Gene₃-lipid complex

24-WELL PLATE TRANSFECTION MAP:

G_WT Wild Type Hef1a-2xKturn-eGFP	G_M Mutant Hef1a-2xKturn-eGFP	//////	//////	-ve control Dummy DNA With Lipofectamine
G_WT Wild Type Hef1a-2xKturn-eGFP	G_M Mutant Hef1a-2xKturn-eGFP	//////	//////	-ve control Dummy DNA With Lipofectamine
		//////	//////	-ve/-ve control No DNA No Lipofectamine
		//////	//////	-ve/-ve control No DNA No Lipofectamine

RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

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