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Experiment #1

GOAL: Test the strength of L7ae

3 incomplete 7/14/14: Seed cells 22 incomplete 7/15/14:  Transfect Transfect MAV1212-hEF1a-eBFP2 (___ ng/uL), Mutant hEF1a-2xKturn-eGFP (___ ng/uL) and Wild Type hEF1a-2xKturn-eGFP (___ ng/uL) into HEK cells 0 incomplete 7/16/14: Flow Cytometer

 

  • DNA Dilution: ___uL MAV1212-hEF1a-eBFP2, ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP


24-WELL PLATE TRANSFECTION MAP:

(B)1

  • MAV1212-hEF1a-eBFP2

(B)2

  • MAV1212-hEF1a-eBFP2
////////////////////////

(GM)1

  • Mutant Hef1a-2xKturn-eGFP

(GM)2

  • Mutant Hef1a-2xKturn-eGFP

(GM + B)1

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GM + B)2

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GM + B + L)1

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEF1a-L7ae

(GM + B + L)2

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEFa-L7ae

(GWT)1

  • Wild Type Hef1a-2xKturn-eGFP

(GWT)2

  • Wild Type Hef1a-2xKturn-eGFP


(GM + B)1

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GM + B)2

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GM + B + L)1

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEFa1-L7ae

(GM + B + L)2

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEF1a-L7ae

Neg Ctrl

  • Dummy DNA
  • With Lipofectamine


Neg Ctrl

  • Dummy DNA
  • With Lipofectamine


Neg/Neg Ctrl

  • No DNA
  • No Lipofectamin

Neg/Neg Ctrl

  • No DNA
  • No Lipofectamine
////////////


RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

Experiment #2

GOAL: Test the strength of TAL14

2 incomplete Day 1: Seed cells 1 incomplete Day 2: Transfect Promoter:Gene1 (___ ng/uL - transfection marker), Promoter:Gene2 (___ ng/uL) and Promoter:Gene3 (___ ng/uL) into HEK cells 7 incomplete Day 4: Flow Cytometer
  • DNA Dilution: ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP, ___ uL hEF1a-L7ae
  • (Eg)x: add _:_:_
    1. __uL Promoter:Gene1-lipid complex
    2. __uL Promoter:Gene2-lipid complex
    3. __uL Promoter:Gene3-lipid complex


24-WELL PLATE TRANSFECTION MAP:

-ve control
      
      

 

     

Neg Ctrl

GWT

  • Wild Type Hef1a-2xKturn-eGFP

GM

  • Mutant Hef1a-2xKturn-eGFP
 ////////////-ve control
  • Dummy DNA
  • With Lipofectamine

GWT

  • Wild Type Hef1a-2xKturn-eGFP

GM

  • Mutant Hef1a-2xKturn-eGFP
 ////////////

  • Dummy DNA
  • With Lipofectamine
 

Neg Ctrl

 
  • Dummy DNA
 
  • With Lipofectamine
////////////

Neg/Neg Ctrl

-ve/-ve control
  • No DNA
  • No LipofectamineLipofectamin
 Neg/Neg Ctrl
    		  ////////////-ve/-ve control
    • No DNA
    • No Lipofectamine
    		  

    RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

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