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Experiment #1

GOAL: Test the strength of L7ae

3 incomplete 7/14/14: Seed cells 22 incomplete 7/15/14: Transfect MAV1212-hEF1a-eBFP2 (___ ng/uL), Mutant hEF1a-2xKturn-eGFP (___ ng/uL) and Wild Type hEF1a-2xKturn-eGFP (___ ng/uL) into HEK cells 0 incomplete 7/16/14: Flow Cytometer

 

  • DNA Dilution: ___uL MAV1212-hEF1a-eBFP2, ___ uL Mutant Hef1a-2xKturn-eGFP, ___ uL Wild Type Hef1a-2xKturn-eGFP


24-WELL PLATE TRANSFECTION MAP:

(B)1

  • MAV1212-hEF1a-eBFP2

(B)2

  • MAV1212-hEF1a-eBFP2
////////////////////////

(GM)1

  • Mutant Hef1a-2xKturn-eGFP

(GM)2

  • Mutant Hef1a-2xKturn-eGFP

(GM + B)1

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GM + B)2

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GM + B + L)1

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEF1a-L7ae

(GM + B + L)2

  • Mutant Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEFa-L7ae

(GWT)1

  • Wild Type Hef1a-2xKturn-eGFP

(GWT)2

  • Wild Type Hef1a-2xKturn-eGFP


(GWT + B)1

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GWT + B)2

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2

(GWT + B + L)1

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEFa1-L7ae

(GWT + B + L)2

  • Wild Type Hef1a-2xKturn-eGFP
  • MAV1212-hEF1a-eBFP2
  • hEF1a-L7ae

Neg Ctrl

  • Dummy DNA
  • With Lipofectamine


Neg Ctrl

  • Dummy DNA
  • With Lipofectamine


Neg/Neg Ctrl

  • No DNA
  • No Lipofectamin

Neg/Neg Ctrl

  • No DNA
  • No Lipofectamine
////////////


RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

Experiment #2

GOAL: Test the strength of TAL14

Day 1: Seed cells

Day 2: Transfect Promoter:Gene1 (___ ng/uL - transfection marker), Promoter:Gene2 (___ ng/uL) and Promoter:Gene3 (___ ng/uL) into HEK cells

Day 4: Flow Cytometer

DNA Dilution: ___ uL hEF1a-eBFP2, ___ uL UAS-GAL4-TAL14-eYFP, ___ uL hEF1a-GAL4VP16, ___ uL hEF1a-TAL14


24-WELL PLATE TRANSFECTION MAP:

(B)1

  • MAV1212-hEF1a-eBFP2

(B)2

  • MAV1212-hEF1a-eBFP2
//////////////////

Neg Ctrl

  • Dummy DNA
  • With Lipofectamine
  • MAV1212-hEF1a-eBFP2
  • 5xUAS/4xTAL14bs-eYFP
  • MAV1212-hEF1a-eBFP2
  • 5xUAS/4xTAL14bs-eYFP
//////////////////

Neg Ctrl

  • Dummy DNA
  • With Lipofectamine
  • MAV1212-hEF1a-eBFP2
  • 5xUAS/4xTAL14bs-eYFP
  • hEF1a-Gal4VP16
  • MAV1212-hEF1a-eBFP2
  • 5xUAS/4xTAL14bs-eYFP
  • hEF1a-Gal4VP16
//////////////////

Neg/Neg Ctrl

  • No DNA
  • No Lipofectamin
  • MAV1212-hEF1a-eBFP2
  • 5xUAS/4xTAL14bs-eYFP
  • hEF1a-Gal4VP16
  • hEF1a-TAL14
  • MAV1212-hEF1a-eBFP2
  • 5xUAS/4xTAL14bs-eYFP
  • hEF1a-Gal4VP16
  • hEF1a-TAL14
//////

 

////////////Neg/Neg Ctrl
  • No DNA
  • No Lipofectamine

RESULT: Brief description of experimental outcome (mean transfection efficiency and implications). Link to results page with data analysis.

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