Well | (HEK293 cells)1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 |
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Beta-Amyloid Concentration (nM) | 0 | 0 | 0 | 0.1 | 0.1 | 0.5 | 0.5 | 1 | 1 | 2.5 | 2.5 | 5 | 5 | 10 | 10 | 25 | 25 | 50 | 50 | 100 | 100 | 1000 | 1000 |
1 HEK293 | Well 2 HEK293 Dummy DNA | Well 3 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate | Well 4 HEK293 anti IgM | Well 5 HEK293 Dummy DNA anti IgM | Well 6 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate anti IgM |
Well 7 HEK293 1000 nm abeta | Well 8 HEK293 Dummy DNA 1000 nm abeta | Well 9 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 1000 nm abeta | Well 10 EMPTY | Well 11 EMPTY | Well 12 EMPTY |
Well 13 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 0 nm abeta | Well 14 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate .1 nm abeta | Well 15 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate .25 nm abeta | Well 16 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate .5 nm abeta | Well 17 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 1 nm abeta | Well 18 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 2.5 nm abeta |
Well 19 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 5 nm abeta | Well 20 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 10 nm abeta | Well 21 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 25 nm abeta | Well 22 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 50 nm abeta | Well 23 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 100 nm abeta | Well 24 HEK293 hEF1a: GmabL hEF1a: GmabH hEF1a: CD79A hEF1a: CD79B hEF1a: mKate 1000 nm abeta | Transfected? | no | dummy | yes | no | yes | no | yes | no | yes | no | yes | no | yes | no | yes | no | yes | no | yes | no | yes | no | yes |