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titleParts Needed
Parts
pEXPR hEF1a: Gmab Heavy
pEXPR hEF1a: Gmab Light
pEXPR hEF1A: CD79A+stop
pEXPR hEF1a: CD79B+stop
pEXPR hEF1a: eBFP
anti-IgM
beta amyloid
streptavidin-fluorophore
Expand
titleSetup
(HEK293 cells)yes

Well

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Beta-Amyloid Concentration (nM)0000.10.10.50.5112.52.55510102525505010010010001000

1

HEK293

Well 2

HEK293

Dummy DNA

Well 3

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

Well 4

HEK293

anti IgM

Well 5

HEK293

Dummy DNA

anti IgM

Well 6

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

anti IgM

Well 7

HEK293

1000 nm abeta

Well 8

HEK293

Dummy DNA
1000 nm abeta

Well 9

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

1000 nm abeta

Well 10

EMPTY

Well 11

EMPTY

Well 12

EMPTY

Well 13

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

0 nm abeta

Well 14

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

.1 nm abeta

Well 15

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

.25 nm abeta

Well 16

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

.5 nm abeta

Well 17

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

1 nm abeta

Well 18

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

2.5 nm abeta

Well 19

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

5 nm abeta

Well 20

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

10 nm abeta

Well 21

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

25 nm abeta

Well 22

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

50 nm abeta

Well 23

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

100 nm abeta

Well 24

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: mKate

1000 nm abeta
Transfected?nodummyyesnoyesnoyesnoyesnoyesnoyesnoyesnoyesnoyesnoyesno
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titleExpected Results

 

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