Testing effectiveness of miRNA1–miRNA4 in down-regulating BACE1 expression Repeat for miRNA2, miRNA3, and miRNA4 (same arrangement of wells as shown here, just substituting out "miRNA1" for the appropriate miRNA) - Hef1a: eBFP 1000ng
Negative Control
| - Hef1a: eBFP 500ng
- Hef1a: BACE1 500ng
Positive Control | | | | | - Hef1a: eBFP 333ng
- TRE: miRNA1 333ng
- Hef1a: rtTA 333ng
0 nM Dox | - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
0 nM Dox | - Hef1a: eBFP 333ng
- TRE: miRNA1 333ng
- Hef1a: rtTA 333ng
1 nM Dox | - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
1 nM Dox | - Hef1a: eBFP 333ng
- TRE: miRNA1 333ng
- Hef1a: rtTA 333ng
10 nM Dox | - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
10 nM Dox | - Hef1a: eBFP 333ng
- TRE: miRNA1 333ng
- Hef1a: rtTA 333ng
100 nM Dox | - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
100 nM Dox | - Hef1a: eBFP 333ng
- TRE: miRNA1 333ng
- Hef1a: rtTA 333ng
1000 nM Dox | - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
1000 nM Dox | - Hef1a: eBFP 333ng
- TRE: miRNA1 333ng
- Hef1a: rtTA 333ng
2000 nM Dox | - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
2000 nM Dox | | | | | | | |
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