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titleParts Needed
Parts
pEXPR hEF1a: Gmab Heavy
pEXPR hEF1a: Gmab Light
pEXPR hEF1A: CD79A+stop
pEXPR hEF1a: CD79B+stop
pEXPR hEF1a: eBFP
anti-IgM
beta amyloid
streptavidin-fluorophore
Expand
titleSetup

 

 One with oligomers one with monomers

Well 1

HEK293

Well 2

HEK293

Dummy DNA

hEF1a: eYFP

Well 3

HEK293

Dummy DNA

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

Well 4

HEK293

 

Well 5

HEK293

Dummy DNA

hEF1a: eYFP


Well 6

HEK293

Dummy DNA

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP


Well 7

HEK293

Well 8

HEK293

Dummy DNA

hEF1a: eYFP


Well 9

HEK293

Dummy DNA

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP


Well 10

HEK293

Well 11

HEK293

Dummy DNA

hEF1a: eYFP


Well 12

HEK293

Dummy DNA

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP


Well 13

HEK293

Well 14

HEK293

Dummy DNA

hEF1a: eYFP


Well 15

HEK293

Dummy DNA

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP


Well 16

HEK293

Well 17

HEK293

Dummy DNA

hEF1a: eYFP


Well 18

HEK293

Dummy DNA

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP


Well 19

EMPTY

Well 20

EMPTY

Well 21

EMPTY

Well 22

EMPTY

Well 23

EMPTY

Well 24

EMPTY

 

 

 

Well 7?

HEK293

hEF1a: eYFP

Dummy DNA
1000 nM abeta

Well 1

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0 nM abeta oligomer

Well 2

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0.1 nM abeta oligomer

Well 3

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0.25 nM abeta oligomer

Well 4

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0.5 nM abeta oligomer

Well 5

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1 nM abeta oligomer1000 nM abeta

Well 6

HEK293

Dummy DNA

1000 nM abeta

EMPTY

EMPTYEMPTYEMPTY

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

2.5 nM abeta oligomer

Well 7

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

5 nM abeta oligomer

Well 8

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

10 nM abeta oligomer

Well 9

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

25 nM abeta oligomer

Well 10

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

50 nM abeta oligomer

Well 11

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

100 nM abeta oligomer

Well 12

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1000 nM abeta oligomer

EMPTY

Well 13

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

0 nm nM abeta oligomer

Well 14

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

.1 nm nM abeta

Well 15

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

.25 nm nM abeta

Well 16

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

.5 nm nM abeta

Well 17

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1 nm nM abeta

Well 18

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

2.5 nm nM abeta

Well 19

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

5 nM abeta

Well 20

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

10 nM abeta

Well 21

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

25 nM abeta

Well 22

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

50 nM abeta

Well 23

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

100 nM abeta

Well 24

HEK293

hEF1a: GmabL

hEF1a: GmabH

hEF1a: CD79A

hEF1a: CD79B

hEF1a: eYFP

1000 nM abeta
Expand
titleExpected Results

 

...