Testing effectiveness of miRNA1–miRNA4 in down-regulating BACE1 expression Repeat for miRNA2, miRNA3, and miRNA4 (same arrangement of wells as shown here, just substituting out "miRNA1" for the appropriate miRNA) - Hef1a: eBFP 1000ng
Negative 250ng - Dummy DNA 750ng
Positive Control
| - Hef1a: eBFP 500ng 250ng
- Hef1a: BACE1 250ng
- Dummy DNA 500ng
Positive Control | | | | Negative Control | | 333ng | 333ng | 333ng- 250ng
- Dummy DNA 250ng
0 nM Dox
| - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
0 nM Dox | | 333ng | 333ng | 333ng
1 - 250ng
- Dummy DNA 250ng
1000 nM Dox
| - Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
| 1 1000 nM Dox | | | | | | | | | | | | | | | | | | | | | Hef1a: eBFP 333ngTRE: miRNA1 333ngHef1a: rtTA 333ng
5 nM Dox- Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
5 nM Dox | Hef1a: eBFP 333ngTRE: miRNA1 333ngHef1a: rtTA 333ng
10 nM Dox- Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
10 nM Dox | Hef1a: eBFP 333ngTRE: miRNA1 333ngHef1a: rtTA 333ng
50 nM Dox- Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
50 nM Dox | Hef1a: eBFP 333ngTRE: miRNA1 333ngHef1a: rtTA 333ng
100 nM Dox- Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
100 nM Dox | Hef1a: eBFP 333ngTRE: miRNA1 333ngHef1a: rtTA 333ng
500 nM Dox- Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
500 nM Dox | Hef1a: eBFP 333ngTRE: miRNA1 333ngHef1a: rtTA 333ng
1000 nM Dox- Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
1000 nM Dox | Hef1a: eBFP 333ngTRE: miRNA1 333ngHef1a: rtTA 333ng
2000 nM Dox- Hef1a: eBFP 250ng
- TRE: miRNA1 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
2000 nM Dox |
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