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Testing effectiveness of miRNA1–miRNA4 in down-regulating BACE1 expression

Repeat for miRNA2, miRNA3, and miRNA4 (same arrangement of wells as shown here, just substituting out "miRNA1" for the appropriate miRNA)

    • Hef1a: eBFP 250ng
    • Dummy DNA 750ng


      Positive Control
    • Hef1a: eBFP 250ng
    • Hef1a: BACE1 250ng
    • Dummy DNA 500ng

Negative Control

    • Hef1a: eBFP 250ng
    • TRE: miRNA1 250ng
    • Hef1a: rtTA 250ng
    • Dummy DNA 250ng

      0 nM Dox
    • Hef1a: eBFP 250ng
    • TRE: miRNA1 250ng
    • Hef1a: rtTA 250ng
    • Hef1a: BACE1 250ng

0 nM Dox

    • Hef1a: eBFP 250ng
    • TRE: miRNA1 250ng
    • Hef1a: rtTA 250ng
    • Dummy DNA 250ng

      1000 nM Dox
    • Hef1a: eBFP 250ng
    • TRE: miRNA1 250ng
    • Hef1a: rtTA 250ng
    • Hef1a: BACE1 250ng

1000 nM Dox

 

     
      
      

Testing inducible expression of BACE2