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We expect to find clear membrane localization of red alexafluor conjugated streptaviding bound to beta amyloid bound to the BCR antibody. This will give us red halos around yellow cells with potentially blue nuclei due to DAPI staining. Moreover we expect a Kd that saturates at approximately 100 nM abeta based on the specifications of Gantenerumab. We are unsure whether the antibody will bind both monmerized and oligomerized beta amyloid. |
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(http://www.renyi.hu/~stipsicz/skin/Flow_cytometry_book/Ch_17.pdf p.16-19 - protocol for yeast using c-myc as transfection marker) |
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