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Cloak

Transfection notes:

  • 1ug Hef1a:BACE1
  • 1ug TRE:BACE2
  • 2ug Hef1a: rtTA
  • 3ug Hef1a:eBFP
  • 3ug Dummy DNA
  • 500uL diluted OptiMEM-lipofectamine

Neuronal research–primary neuron cultures and APPSwe HEK293:

  • ease of culturing
  • accuracy of models
  • where do we get them
  • pros/cons of use

 

AL -8/5

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miRG1 (BFP transfection marker) w/ red channel cranked

miRG1 + BACE1 (BFP transfection marker) w/ red channel cranked