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Treatment

A3

Hef1a: eBFP 250ng

TRE: miRNA1 250ng

Hef1a: rtTA 250ng

Dummy DNA 250ng
0 nM Dox

A5

Hef1a: eBFP 250ng

TRE: miRNA1 250ng

Hef1a: rtTA 250ng

Dummy DNA 250ng
1000 nM DoxTesting effectiveness of miRNA1–miRNA4 in down-regulating BACE1 expression v1

Cloak
Testing effectiveness of miRNA1–miRNA4 in down-regulating BACE1 expression v1 Repeat for miRNA2, miRNA3, and miRNA4 (same arrangement of wells as shown here, just substituting out "miRNA1" for the appropriate miRNA) A1 Hef1a: eBFP *250*ng Dummy DNA 750ng Positive Control	A2 Hef1a: eBFP *250ng Hef1a: BACE1 250ng Dummy DNA 500ng* Negative Control	A4 Hef1a: eBFP 250ng TRE: miRNA1 250ng Hef1a: rtTA 250ng Hef1a: BACE1 250ng 0 nM Dox	A6 Hef1a: eBFP 250ng TRE: miRNA1 250ng Hef1a: rtTA 250ng Hef1a: BACE1 250ng 1000 nM Dox

A1 Hef1a: eBFP *250*ng Dummy DNA 750ng Positive Control	A2 Hef1a: eBFP *250ng Hef1a: BACE1 250ng Dummy DNA 500ng* Negative Control	A3 Hef1a: eBFP *250ng TRE: miRNA1-mKATE250ng Hef1a: rtTA 250*ng Dummy DNA 250ng 0 nM Dox	A4 Hef1a: eBFP 250ng TRE: miRNA1-mKATE 250ng Hef1a: rtTA 250ng Hef1a: BACE1 250ng 0 nM Dox	A5 Hef1a: eBFP *250ng* TRE: miRNA1-mKATE *250ng Hef1a: rtTA 250ng Dummy DNA 250ng* 1000 nM Dox	A6 Hef1a: eBFP 250ng TRE: miRNA1-mKATE 250ng Hef1a: rtTA 250ng Hef1a: BACE1 250ng 1000 nM Dox
A7 Hef1a:mKATE 250ng Hef1a:BFP 250ng Dummy DNA 500ng

Testing inducible expression of BACE2

B1 Hef1a:eBFP 333ng Dummy DNA 666ng Negative Control	B2 Hef1a:eBFP 333ng Hef1a:rtTA 333ng TRE:BACE2 333ng 0 nM Dox	B3 Hef1a:eBFP 333ng Hef1a:rtTA 333ng TRE:BACE2 333ng 1000 nM Dox	B4 No DNA (untransfected) Double negative control

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