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Well 1

HEK293

 

Well 2

HEK 293Anti

 

UAS: mKate-IgM

Well 3

HEK293

 

Well 4

 HEK293

Well 5  

HEK293

Well 6  

HEK293

Well 7

 

Well 8

 

Well 9

 

Well 10

 

Well 11Well 12

Well 7

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

0nM dox

Well 8

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

1nM dox

Well 9

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

10nM dox

Well 10

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

100nM dox

Well 11

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

1000nM dox

Well 12

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

2000nM dox

Well 13

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

0nM dox

Anti-IgM 

Well 14

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

1nM dox

Anti-IgM 

Well 15

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

10nM dox

Anti-IgM 

Well 16

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

100nM dox

Anti-IgM 

Well 17

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

1000nM dox

Anti-IgM 

Well 18

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

2000nM doxAnti-IgM