Testing effectiveness of miRNA1–miRNA4 in down-regulating BACE1 expression v1 A1 - Hef1a: eBFP 250ng
- Dummy DNA 750ng
Positive Control
| A2 - Hef1a: eBFP 250ng
- Hef1a: BACE1 250ng
- Dummy DNA 500ng
Negative Control | A3 - Hef1a: eBFP 250ng
- TRE: miRNA1-mKATE250ng
- Hef1a: rtTA 250ng
- Dummy DNA 250ng
0 nM Dox
| A4 - Hef1a: eBFP 250ng
- TRE: miRNA1-mKATE 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
0 nM Dox | A5 - Hef1a: eBFP 250ng
- TRE: miRNA1-mKATE 250ng
- Hef1a: rtTA 250ng
- Dummy DNA 250ng
1000 nM Dox
| A6 - Hef1a: eBFP 250ng
- TRE: miRNA1-mKATE 250ng
- Hef1a: rtTA 250ng
- Hef1a: BACE1 250ng
1000 nM Dox | A7 - Hef1a:mKATE 250ng
- Hef1a:BFP 250ng
- Dummy DNA 500ng
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Testing inducible expression of BACE2 B1 - Hef1a:eBFP 333ng
- Dummy DNA 666ng
Negative Control | B2 - Hef1a:eBFP 333ng
- Hef1a:rtTA 333ng
- TRE:BACE2 333ng
0 nM Dox | B3 - Hef1a:eBFP 333ng
- Hef1a:rtTA 333ng
- TRE:BACE2 333ng
1000 nM Dox | B4 Double negative control | | | | | | | | | | | | | | | | | | | | | | | |
Transfection: Week of 8/11 A1 - Hef1a: eBFP 250ng
- Dummy DNA 750ng
Negative BACE1 Control
| A2 - Hef1a: eBFP 250ng
- Hef1a: BACE1 250ng
- Dummy DNA 500ng
Positive BACE1 Control | A3 - Hef1a:mKATE 250ng
- Hef1a:BFP 250ng
- Dummy DNA 500ng
mKATE control | A4 - Hef1:miRNAg1 250ng
- Hef1a:BFP 250ng
- Dummy DNA 500ng
miRNA1g control | | | | | | | | | | | | | | | | | | | | B1 - Hef1a:eBFP 333ng
- Dummy DNA 666ng
Negative Control | B2 - Hef1a:eBFP 333ng
- Hef1a:rtTA 333ng
- TRE:BACE2 333ng
0 nM Dox | B3 - Hef1a:eBFP 333ng
- Hef1a:rtTA 333ng
- TRE:BACE2 333ng
1000 nM Dox | B4 Double negative control |
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