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titleTest 4
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titleDescription

 

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titlePurpose

 

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titleParts Needed

pEXPR hEF1a: Gmab Light

pEXPR hEF1a: Gmab Heavy

pEXPR hEF1a: CD79A

pEXPR hEF1a: CD79B

ALSO NEED:

Anti IgM primary antibody fused to a yellow alexa dye

B cells

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titleSetup

PLATE 1

Well 1

B-Cells

Well 2

B-Cells

Anti IgM

Well 3

Permeablized B-Cells

Anti IgM

Well 4

HEK293

Well 5

HEK293

100 mKate

400 Dummy

Well 6

EMPTY

Well 7

B-Cells

Well 8

B-Cells

Anti IgM

Well 9

Permeablized B-Cells

Anti IgM

Well 10

HEK293

Well 11

HEK293

100 mKate

400 Dummy

Well 12

EMPTY

Well 13 

HEK293

100 Gmab H

100 Gmab L

100 CD79A

100 CD79B

100 mKate

Anti IgM

Well 814 

HEK293

50 ng Gmab H

50 ng Gmab L

50 ng CD79A

50 ng CD79B

100 ng mKate

200 ng mKate

Anti IgM

Well 915

HEK293

25 ng Gmab H

25 ng Gmab L

25 ngCD79AngCD79A

25 ng CD79B

100 ng mKate

300 ng Dummy DNA

Anti IgM

Well 1016

HEK293

12.5 ng Gmab H

12.5 ng Gmab L

12.5 ng CD79A

12.5 ng CD79B

100 ng mKate

350 ng Dummy DNA

Anti IgM

 

Well 1117

EMPTY

 

Well 1218

EMPTY

Well 13 19

HEK293

100 Gmab H

100 Gmab L

100 CD79A

100 CD79B

100 mKate

Anti IgM

Well 14 20

HEK293

50 ng Gmab H

50 ng Gmab L

50 ng CD79A

50 ng CD79B

100 ng mKate

200 ng mKate

Anti IgM

Well 1521

HEK293

25 ng Gmab H

25 ng Gmab L

25 ngCD79A

25 ng CD79B

100 ng mKate

300 ng Dummy DNA

Anti IgM

Well 1622

HEK293

12.5 ng Gmab H

12.5 ng Gmab L

12.5 ng CD79A

12.5 ng CD79B

100 ng mKate

350 ng Dummy DNA

Anti IgM

 

Well 17

EMPTY

 

Well 18

EMPTY

Well 19 

EMPTY

Well 20

EMPTY

Well 21

EMPTY

Well 22

EMPTY

 

Well 23

EMPTY

 

Well 24 

EMPTY

 

 

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titleProtocol

 

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titleData

 

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titleAnalysis