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Well 1 | Well 2 | Well 3 | Well 4 | Well 5 | Well 6 |
|---|---|---|---|---|---|
untransfected (calibration) | eBFP bleed through control | leaky mKate control | TEVp control (nonspecific G4 cleavage) | TEVp control (nonspecific G4 cleavage) TEVp control | (nonspecific G4 cleavage) |
HEK293
| HEK293 100 ug hEF1a: mKateeBFP 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A 100 ug CD79B 100 ug TRE: Syk-TEVp 100 ug Lyn
| HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A 100 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFPmKate 100 ug Lyn | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B 100 ug UAS: eYFP mKate
| HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A100 ug CD79B-TCS-G4 100 ug UAS: eYFPug CD79B
100 ug HEK293 100 ug hEF1a100 ug UAS: mKate 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B-TCS-G4 100 ug UAS: eYFP anti-IgM |
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| Well 7 | Well 8 | Well 9 | Well 10 | Well 11 | Well 12 |
HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox
| HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 50 ug GmabH 50 ug GmabL 50 ug CD79A-TCS-G4 50 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 25 ug GmabH 25 ug GmabL 25 ug CD79A-TCS-G4 25 ug CD79B 100 ug : Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 12.5 ug GmabH 12.5 ug GmabL 12.5 ug CD79A-TCS-G4 12.5 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 6.25 ug GmabH 6.25 ug GmabL 6.25 ug CD79A-TCS-G4 6.25 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 3.125 ug GmabH 3.125 ug GmabL 3.125 ug CD79A-TCS-G4 3.125 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox |
| Well 13 | Well 14 | Well 15 | Well 16 | Well 17 | Well 18 |
HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A100 ug CD79Bug CD79A-TCS-G4 100 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 50 ug GmabH 50 ug GmabL 50 ug CD79A50 ug CD79Bug CD79A-TCS-G4 50 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 25 ug GmabH 25 ug GmabL 25 ug CD79A25 ug CD79Bug CD79A-TCS-G4 25 ug CD79B 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 12.5 ug GmabH 12.5 ug GmabL 12.5 ug CD79Aug CD79A-TCS-G4 12.5 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 6.25 ug GmabH 6.25 ug GmabL 6.25 ug CD79Aug CD79A-TCS-G4 6.25 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox | HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 3.125 ug GmabH 3.125 ug GmabL 3.125 ug CD79Aug CD79A-TCS-G4 3.125 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox
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| Well 19 | Well 20 | Well 21 | Well 22 | Well 23 | Well 24 |
100 ug HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 100 ug GmabH 100 ug GmabL 100 ug CD79A-TCS-G4 100 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox
| 100 ugHEK293 100 ughEF1a: mKate eBFP 100 ughEF1a: rtTA 50 ug GmabH 50 ug GmabL 50 ug CD79A-TCS-G4 50 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox
| 100 ug HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 25 ug GmabH 25 ug GmabL 25 ug CD79A-TCS-G4 25 ug CD79B-TCS-G4 100 ugTRE: Syk-TEVp 100 ug UAS: eYFP mKate 10nM dox
| 100 ug HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 12.5 ug GmabH 12.5 ug GmabL 12.5 ug CD79A-TCS-G4 12.5 ug CD79B-TCS-G4 100 ugTRE: Syk-TEVp 100 ugUAS100 ug UAS: eYFP mKate 10 nM dox
| 100 ug HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 6.25 ug GmabH 6.25 ug GmabL 6.25 ug CD79A-TCS-G4 6.25 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox
| 100 ug HEK293 100 ug hEF1a: mKate eBFP 100 ug hEF1a: rtTA 3.125 ug GmabH 3.125 ug GmabL 3.125 ug CD79A-TCS-G4 3.125 ug CD79B-TCS-G4 100 ug TRE: Syk-TEVp 100 ug UAS: eYFP mKate 10 nM dox |