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  • Getting another organism to take away the byproducts that hinder hydrogen production
    • combining dark fermentation and photo fermentation - Combined fermentation
    • getting an organism to consume the acetate
  • containment - making the bacteria dependent on each other to survive
  • protein scaffolding to increase metabolic flux
  • making recombinant proteins
  • one bacteria degrading proteins/complex sugars that the other can use for production (http://www.sciencedirect.com/science/article/pii/S0960852414017301)

Important hydrogen production review article: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757257/

BacteriaInputs/Required EnzymesOutputPaper
E. Coli
  • Formate (from pyruvate which needs PFL)
  • FHL (formate hydrogenlyase)
  
Geobacter  http://aem.asm.org/content/78/21/7645.full
Rhodobacter
  • Malic acid
  • Light (photo reaction)
  • nitrogenase
  • hydrogenase
  • can be supplemented by malate
 

http://www.iaeng.org/publication/WCECS2007/WCECS2007_pp141-145.pdf

http://www.sciencedirect.com/science/article/pii/S0360319902001271

Enterobacter   
Caldicellulosiruptor saccharolyticus
  • H2
  • Acetate
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003633/