...
After fixation, most human and mouse tissues and cells are no longer pathogenic and therefore Risk Group 1 and may be observed under standard BL1 laboratory safety conditions. Live non-human and non-primate specimens such as mouse cell lines that cannot possibly contain human pathogens are also BL1.NOTICE_DANGER
Unfixed samples that include ANY human tissue or live human or primate primary cells or cell lines are considered Risk Group 2 agents and require BL2-level imaging. Imaging of live Risk Group 2 (BL2) samples is now possible, but is strictly controlled. Users wishing to image BL2 samples must read the [BL2 Safety Policies](safety-bl2.md)
...
Please check with core personnel if you are unsure if your sample is BL1 or BL2.
Core Safety Policies
General Safety
...
- Report injuries or spills immediately to core staff.
- Report equipment issues or damage immediately to core staff. We can't fix what we don't know about!
- Risk Group 2 (BL2) work requires elevated decontamination procedures (see below).
BL1 and BL2 signs
...
Pay attention to BL1 and BL2 signs outside of each microscope room.
- Fixed specimens can be viewed in rooms designated as BL1.
- Do not enter a room with a BL2 sign on the door unless you have had bloodborne pathogen training.
- BL2 specimens such as live human-derived cells require the temporary raising of a room to BL2 status with proper signage and decontamination at the end of your session. See below.
- BL2+ specimens such as cells with live lentivirus are not permitted in the KIMCF.
...