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- Determine whether your sample is BL1 or BL2 (see below). Ask us if unsure!
- BL2 sign and extra preparation are required while imaging BL2 samples
- Don't pipette or add to your samples on the microscope stage!
- Use our local BL2 manipulation area for that
- Transport samples in airtight secondary containers with latching lids.
- MIT users must amend their BRR to include the microscopy core.
- You need ~30 min of additional training to image BL2 samples!
- You must bring your PPE and secondary container for BL2 training! We will postpone your training if you do not bring these items.
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After fixation, most human and mouse tissues and cells are no longer pathogenic and therefore Risk Group 1. Fixed samples may be observed under standard BL1 laboratory safety conditions. Live non-human specimens such as mouse cell lines that do NOT contain human pathogens are also BL1.
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MIT researchers (internal users) must have approval under their biological research registration (BRR) for use of the materials. BRR status will be verified and you must amend your BRR to include the KI-MCF (rooms 76-280 and 76-281) as a location in Section 2. Here is the current BRR amendment form. Please coordinate the amendment with the EHS rep for your home department and CC core staff in your email.
We cannot provide BL2 services to external entities. All BL2 entities MUST have an existing BRR to amend.
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