...
- The segment to be amplified is the right side of a gene coding for YFP (other people are amplifying the left side)
- The goal will be to amplify the right and left sides of the gene and then assemble them together into a functional gene
- Diluted the two primer solutions (forward primer and reverse)
- Added pre-made "PCR Mix" and primers to DNA
- Set PCR machine to the cycle specifications called for in the protocol
3/14/14 & 3/16/14