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added: 1uL of Hef1a (1) @ 5fM 1uL of EBFP @ 5fM 1uL of pDEST 12 @ 10fM 0.5uL of clonase mixed by pipetting, one air bubble in aliquot. left over night at room temperature Purpose: practice |
JA- 6/13
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after both LR reactions (for JA and AL) from 6/12 incubated at room temp for 24 hours, the the plated transformed e. coli were left in the 30 C incubator at 1:00pm
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