...
| Date | Product | Gene | Starting Plasmid | Forward Primer | Reverse Primer | Tm hi/lo (anneal) | Link to Gel Picture |
|---|---|---|---|---|---|---|---|
| 6/23 | Q2-BACE2-QX | BACE2 | pEXPR BACE2 | iGEM 016 BACE2 primer forward | iGEM 017 BACE2 primer reverse | (60) | |
| 6/23 | Q1-eYFP-QX | eYFP | pEXPR TRE:eYFP | iGEM 018 eYFP primer forward | iGEM 019 eYFP primer reverse | (60) | |
| 6/23 | Q1-BACE2-Q2 | BACE2 | pEXPR BACE2 | iGEM 001-2 BACE2_Primer_F | iGEM 002-2 BACE2_Primer_R | (60) | |
| 6/23 | Q2-eYFP-QX | eYFP | pEXPR TRE:eYFP | iGEM 003-2 YFP_Primer_F | iGEM 004-2 YFP_Primer_R | (60) |
Ran PCRs according to protocol on NEB website: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531
...