| Date | Product | Gene | Starting Plasmid | Forward Primer | Reverse Primer | Thermocycler Variables: # cycles / annealing temp / extension time | Link to Gel Picture | Comments / Deviations from Protocol |
|---|---|---|---|---|---|---|---|---|
| 6/23 | Q2-BACE2-QX | BACE2 | pEXPR BACE2 | iGEM 016 BACE2 primer forward | iGEM 017 BACE2 primer reverse | 30 / 60C / 30s | Picture 6/23 | Diluted primers in water, not TE |
| 6/23 | Q1-eYFP-Q2 | eYFP | pEXPR TRE:eYFP | iGEM 018 eYFP primer forward | iGEM 019 eYFP primer reverse | 30 / 60C / 30s | Picture 6/23 | Diluted primers in water, not TE |
| 6/23 | Q1-BACE2-Q2 | BACE2 | pEXPR BACE2 | iGEM 001-2 BACE2_Primer_F | iGEM 002-2 BACE2_Primer_R | 30 / 60C / 30s | Picture 6/23 | Diluted primers in water, not TE |
| 6/23 | Q2-eYFP-QX | eYFP | pEXPR TRE:eYFP | iGEM 003-2 YFP_Primer_F | iGEM 004-2 YFP_Primer_R | 30 / 60C / 30s | Picture 6/23 | Diluted primers in water, not TE |
Ran PCRs according to protocol on NEB website: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531
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