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Date
Product
Gene
Starting Plasmid
Forward Primer
Reverse Primer

Thermocycler Variables: # cycles /

annealing temp / extension time

Gel Picture

Comments / Deviations

from Protocol

6/23Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 primer forwardC-term primerFiGEM 017 BACE2 primer reverseC-term primerR30 / 60C / 30sPicture 6/23Diluted primers in water, not TE
6/23Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP primer forwardN-term primerFiGEM 019 eYFP primer reverseN-term primerR30 / 60C / 30sPicture 6/23 Diluted primers in water, not TE
6/23Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2 _Primer_F N-term primerFiGEM 002-2 BACE2 _Primer_RN-term primerR30 / 60C / 30sPicture 6/23 Diluted primers in water, not TE
6/23Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP _Primer_FC-term primerFiGEM 004-2 YFP _Primer_RC-term primerR30 / 60C / 30sPicture 6/23 Diluted primers in water, not TE

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