Page History

Date	Product	Gene	Starting Plasmid	Forward Primer	Reverse Primer	Thermocycler Variables: #cycles / annealing temp / extension time	Gel Picture	Comments / Deviations from Protocol
6/23	Q2-BACE2-QX	BACE2	pEXPR BACE2	iGEM 016 BACE2 C-term primerF	iGEM 017 BACE2 C-term primerR	30 / 60C / 30s	Picture 6/23	Diluted primers in water, not TE
6/23	Q1-eYFP-Q2	eYFP	pEXPR TRE:eYFP	iGEM 018 eYFP N-term primerF	iGEM 019 eYFP N-term primerR	30 / 60C / 30s	Picture 6/23	Diluted primers in water, not TE
6/23	Q1-BACE2-Q2	BACE2	pEXPR BACE2	iGEM 001-2 BACE2 N-term primerF	iGEM 002-2 BACE2 N-term primerR	30 / 60C / 30s	Picture 6/23	Diluted primers in water, not TE
6/23	Q2-eYFP-QX	eYFP	pEXPR TRE:eYFP	iGEM 003-2 YFP C-term primerF	iGEM 004-2 YFP C-term primerR	30 / 60C / 30s	Picture 6/23	Diluted primers in water, not TE
6/24	Q2-BACE2-QX	BACE2	pEXPR BACE2	iGEM 016 BACE2 C-term primerF	iGEM 017 BACE2 C-term primerR	35 / 56.2C / 30s		Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers
6/24	Q1-eYFP-Q2	eYFP	pEXPR TRE:eYFP	iGEM 018 eYFP N-term primerF	iGEM 019 eYFP N-term primerR	35 / 56.2C / 30s		Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers
6/24	Q1-BACE2-Q2	BACE2	pEXPR BACE2	iGEM 001-2 BACE2 N-term primerF	iGEM 002-2 BACE2 N-term primerR	35 / 56.2C / 30s		Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers
6/24	Q2-eYFP-QX	eYFP	pEXPR TRE:eYFP	iGEM 003-2 YFP C-term primerF	iGEM 004-2 YFP C-term primerR	35 / 56.2C / 30s		Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers

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