How To Please Ron/Brain
- Present about cell models
- COMPLETE PARTS LIST ON WIKI
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miRNA Sensors (Theory)
- Prep experiments
- Contact Jeremy about multiple miRNA target sites chained together
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miRNA Sensors (Lab Work)
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- Gateway Pick colonies for MAV1212
- Miniprep MAV1212
- Keep MAV1212-Hef1a-L7aeeBFP2 (for use WITHOUT target sites)
- Gateway MAV1212-Hef1a-mKate2
MORNING
- Miniprep MAV1212
- Check MAV1212 colonies
- Sequence ccdb region
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- PCR LacZ for low sensors
- PCR annealed oligos
- Run the gel
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- L7ae (for high sensor)
- Gateway MAV1212_Hef1a-eYFP (for use WITH target sites)
- Transform Hef1a, MAV1212 ( ? )