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Date
Product
Gene
Starting Plasmid
Forward Primer
Reverse Primer

Thermocycler Variables: #cycles /

annealing temp / extension time

Gel Picture

Comments / Deviations

from Protocol

6/23Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR30 / 60C / 30sPicture 6/23Diluted primers in water, not TE
6/23Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR30 / 60C / 30sPicture 6/23 Diluted primers in water, not TE
6/23Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR30 / 60C / 30sPicture 6/23 Diluted primers in water, not TE
6/23Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR30 / 60C / 30sPicture 6/23 Diluted primers in water, not TE
6/24Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR35 / 56.2C / 30sPicture 6/24Used 0.6 uL DMSO to reduce risk of hairpins or primer dimersWhen we changed the primer concentration back to default in the NEB Tm calculator as Brian suggested, it gave a lower annealing temp, so we changed the thermo program to reflect this.
6/24Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR35 / 56.2C / 30sPicture 6/24  Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers
6/24Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR35 / 56.2C / 30sPicture 6/24  Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers
6/24Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR35 / 56.2C / 30sPicture 6/24  Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers

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