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6/23 60C / 30sPicture 6/236/24Picture 6/246/24
Date

Lane Order

Product
Gene
Starting Plasmid
Forward Primer
Reverse Primer

Thermocycler Variables: #cycles /

annealing temp / extension time

Gel Picture

Comments / Deviations

from Protocol

6/23 Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR30 / 60C / 30sPicture Diluted primers in water, not TED 6/23
Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR30 / 60C / 30sPicture 6/23 
6/23Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR30 / 60C / 30sPicture 6/23 
6/23Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR30
6/24 6/24Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR35 / 56.2C / 30sPicture 6/24Used 0.6 uL DMSO to reduce risk of hairpins or primer dimers
6/24Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR35 / 56.2C / 30sPicture 6/24  
6/24Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR35 / 56.2C / 30sPicture 6/24  
6/24Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR35 / 56.2C / 30sPicture 6/24  
6/25

ladder | N-term BACE2 | C-term BACE2 | N-term eYFP | C-term eYFP

 

 

Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR35 / 5650.2C / 30s 
Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerRPicture 6/24  
6/24Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerRPicture 6/24  
6/24Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerRPicture 6/24  

Ran PCRs according to protocol on NEB website: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531

...