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6/26
   

 

 

 

 

    
   
Date
Product
Gene
Starting Plasmid
Forward Primer
Reverse Primer

Thermocycler Variables: #cycles /

annealing temp / extension time

Gel Picture
6/23Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR30 / 60C / 30sGel Picture 6/23
Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR
Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR
Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR
6/24Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR35 / 56.2C / 30sGel Picture 6/24
Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR
Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR
Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR
6/25Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR

35 / 50.2C / 30s

 

 

 

 

 

Gel Picture 6/25
Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR
Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR
Q1-eYFP-Q2eYFPpEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR
Q2-eYFP-QXeYFPpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR
6/26


Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR

30 / 55C / 1m

 

 

 

 

 




Gel Picture 6/26
Q2-BACE2-QXBACE2pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR


 Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR
Q1-BACE2-Q2BACE2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR
  
6/27Q2-BACE1-QXQ1-BACE1-Q2BACE2pEXPR BACE1iGEM 014 023 BACE1 NC-term primerFiGEM 015 022 BACE1 NC-term primerR Gel Picture 6/27
Q2-BACE1eYFP-QXBACE2pEXPR BACE1eYFPiGEM 023 BACE1 C012 eYFP  C-term primerFiGEM 022 BACE1 013 eYFP C-term primerR
6/30Q1-eYFP-Q2pEXPR eYFP    
Q1-BACE1-Q2pEXPR BACE1  pEXPR eYFPiGEM 021 eYFP  N-term primerFiGEM 020 eYFP N-term primerRQ2-eYFP-QXeYFPpEXPR eYFPiGEM 012 eYFP  C-term primerFiGEM 013 eYFP C-term primerR

 

 

 

 

Ran PCRs according to protocol on NEB website: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531

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