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LR 06/16:
LR
Transformation 06/18
Pick colonies
Miniprep, digest, sequence
Midiprep
transformation
pick colonies
miniprep
digest + gel
potential mix up in loading the gel, diges+gel repeated
digest + gel
reLR
digest + gel of LR1 with different enzymes
cultured and miniprep LR2
midi culture
midi
Gel
Image Added | 6/23 Image Added | Notes: Band pattern doesn't match either a successful LR or a failed one. Band pattern matches pLac:mKate which was run on the same gel, indicating that samples may be loaded in the wrong order. |
Image Added | Image Added | |
Image Added | Image Added | Perfect. |
Sequencing results