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To prepare, start with 500 ml of Milli Q and add the following:

  • 1.0 g 0g Peptone
  • 0.5 g 5g Yeast Extract
  • 0.15 g MgSO~4~15g MgSO 4
  • 500ul 500 ul 0.5M CaCl~2~CaCl 2

Mix together and autoclave