Page History

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2

HEK 293

Anti-IgM

Well 8

Well 7

Well

1

Well 2

Well 3

Well 4

Well 5

Well 6

untransfected

(calibration)

eBFP bleed through control

leaky mKate control

TEVp control

(nonspecific G4 cleavage)

TEVp control

(nonspecific G4 cleavage)

TEVp control

(nonspecific G4 cleavage)

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Syk-TEVp

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B

TRE: Syk-TEVp

UAS: mKate

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

UAS: mKate

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B-TCS-G4

UAS: mKate

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

UAS: mKate

Well 7

Well 8

Well 9

Well 10

Well 11

Well 12

Well 5

Well 6

Well 7

Well 9

Well 10

Well 11

Well 12

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

0nM dox

Well 8

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

1nM doxWell 9

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

10nM dox

Well 10

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

100nM dox

Well 11

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

1000nM dox

Well 12

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B

TRE: Syk-TEVp

UAS:mKate

2000nM dox

Well 13

Well 14

Well 15

Well 16

Well 17

Well 18

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

0nM dox

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

0nM dox

Anti-IgM

1nM dox

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

10nM doxWell 14

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

100nM dox

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

1nM dox

Anti-IgM

Well 15

1000nM dox

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

2000nM dox

Well 19

Well 20

Well 21

Well 22

Well 23

Well 24

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

10nM dox

Anti-IgM

0nM dox

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

1nM dox

Well 16

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

10nM dox

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

100nM dox

Anti-IgM

Well 17

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

1000nM dox

Anti-IgM

Well 18

HEK293

hEF1a: eBFP

hEF1a: rtTA

GmabH

GmabL

CD79A-TCS-G4

CD79B-TCS-G4

TRE: Syk-TEVp

UAS:mKate

2000nM doxAnti-IgM

	Untransfected	eBFP + Syk-TEVp + BCR (no Gal4VP16)	eBFP + Syk-TEVp + BCR (no Gal4VP16) + UAS:mKate	eBFP + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate	eBFP + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate	eBFP + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate
	Image Added	Image Added Transfection Efficiency: ~20%	Image Added	Image Added	Image Added	Image Added
eBFP + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [0nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [1nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [10nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [100nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [1000nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [2000nM dox]
Image Added	Image Added Cotransfection Efficiency: ~4%	Image Added	Image Added	Image Added	Image Added	Image Added
eBFP + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate	eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [0nM dox]	eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [1nM dox]	eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [10nM dox]	eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [100nM dox]	eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [1000nM dox]	eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [2000nM dox]
Image Added	Image Added Cotransfection Efficiency: ~3.3%	Image Added	Image Added	Image Added	Image Added	Image Added
eBFP + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [0nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [1nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [10nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate 100nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [1000nM dox]	eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [2000nM dox]
Image Added	Image Added Cotransfection efficiency: ~3.3%	Image Added	Image Added	Image Added	Image Added	Image Added

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