Page History

Forward Primer

Date	Product	Gene	Starting Plasmid		Reverse Primer	Thermocycler Variables: #cycles / annealing temp / extension time	Gel Picture
6/23	Q2-BACE2-QX	BACE2	pEXPR BACE2	iGEM 016 BACE2 C-term primerF	iGEM 017 BACE2 C-term primerR	30 / 60C / 30s	Gel Picture 6/23
	Q1-eYFP-Q2	eYFP	pEXPR TRE:eYFP	iGEM 018 eYFP N-term primerF	iGEM 019 eYFP N-term primerR
	Q1-BACE2-Q2	BACE2	pEXPR BACE2	iGEM 001-2 BACE2 N-term primerF	iGEM 002-2 BACE2 N-term primerR
	Q2-eYFP-QXeYFP	pEXPR TRE:eYFPiGEM 003-2 YFP C-term primerF	iGEM 004-2 YFP C-term primerR
6/24	Q2-BACE2-QX	BACE2	pEXPR BACE2iGEM 016 BACE2 C-term primerF	iGEM 017 BACE2 C-term primerR	35 / 56.2C / 30s	Gel Picture 6/24
	Q1-eYFP-Q2	eYFP	pEXPR TRE:eYFP	iGEM 018 eYFP N-term primerF			iGEM 019 eYFP N-term primerR
	Q1-BACE2-Q2	BACE2	pEXPR BACE2	iGEM 001-2 BACE2 N-term primerF			iGEM 002-2 BACE2 N-term primerR
	Q2-eYFP-QX	eYFP	pEXPR TRE:eYFPiGEM 003-2 YFP C-term primerF	iGEM 004-2 YFP C-term primerR
6/25	Q1-BACE2-Q2BACE2	pEXPR BACE2	iGEM 001-2 BACE2 N-term primerF	iGEM 002-2 BACE2 N-term primerR	35 / 50.2C / 30s	Gel Picture 6/25
	Q2-BACE2-QX	BACE2	pEXPR BACE2	iGEM 016 BACE2 C-term primerF			iGEM 017 BACE2 C-term primerR
	Q2-BACE2-QX	BACE2	pEXPR BACE2	iGEM 016 BACE2 C-term primerF			iGEM 017 BACE2 C-term primerR
	Q1-eYFP-Q2	eYFP	pEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR
	Q2-eYFP-QXeYFP	pEXPR TRE:eYFP	iGEM 003-2 YFP C-term primerF	iGEM 004-2 YFP C-term primerR
6/26	Q2-BACE2-QX BACE2 (plus betaine)	pEXPR BACE2	iGEM 016 BACE2 C-term primerF	iGEM 017 BACE2 C-term primerR	30 / 55C / 1m	Gel Picture 6/26
	Q2-BACE2-QX BACE2 (plus DMSO)	pEXPR BACE2iGEM 016
	Q1-BACE2-Q2 (plus betaine)	pEXPR BACE2 C-term primerFiGEM 017 BACE2 C-term primerR
	Q1-BACE2-Q2 (plus DMSO)	pEXPR BACE2	pEXPR BACE2	iGEM 001-2 BACE2 N-term primerF			iGEM 002-2 BACE2 N-term primerR	Q1-BACE2-Q2	BACE2	pEXPR BACE2	iGEM 001-2 BACE2 N-term primerF
6/27	Q2-BACE1-QX	pEXPR BACE1		Gel Picture 6/27
6/27	Q2-eYFP-QX	pEXPR eYFP		Gel Picture 6/27
6/30	Q1-eYFP-Q2	pEXPR eYFP		Gel Picture 6/30
6/30	Q1-BACE1-Q2	pEXPR BACE1		Gel Picture 6/30
7/9	Q2-eYFP-QX (for BACE2)	pEXPR eYFP	35 / 58C / 1m with both DMSO and Betaine	Gel Picture 7/9
	Q1-eYFP-Q2 (for BACE2)	pEXPR eYFP
	Q2-eYFP-QX (for BACE1)	pEXPR eYFP
	Q2-BACE2-QX	pEXPR BACE2
	Q1-BACE2-QX	pEXPR BACE2
	Q1-BACE1-QX	pEXPR BACE1
7/10	(miRNA generator stuff)		35 / 58C / 1m with both DMSO and Betaine	Gel Picture 7/10
7/10	(20 PCR products in all)		35 / 58C / 1m with both DMSO and Betaine	Gel Picture 7/10
7/11	(miRNA generator stuff)		30 / 61C / 30s	Gel Picture 7/11
7/11	(12 PCR products in all)		30 / 61C / 30s	Gel Picture 7/11
7/16	(miRNA generator stuff)		30 / 63C / 30s	Gel Picture 7/16
	(12 PCR products in all)	iGEM 002-2 BACE2 N-term primerR

Ran PCRs according to protocol on NEB website: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531

...

Child pages

Versions Compared

Old Version 20

New Version Current

Key