...
Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
| Experiment | Expected Results | Controls | Protocols | Parts | Completed? |
|---|---|---|---|---|---|
| Assay down-regulation of BACE1 by U6/miRNA | |||||
| Assay transcription factor's effectiveness in promoting expression of miRNA/BACE2 | |||||
Purchases
Experiments
Seeding 24-well Plate......Transfection......FACS
| Experiment |
|---|
Demonstrate effectiveness of miRNA in down-regulating BACE1 |
Demonstrate inducible upregulation of BACE2-YFP |
Purchases
| Item | Link | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140 | ||||||||
| BACE1 | |||||||||
| Item | Link | Purchased? | |||||||
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=4116678236 | BACE1 (mouse) | |||||||
| Bace2 activity assay | https://www. | addgeneanaspec. | orgcom/ | 26736/products/product.asp?id=57836 | |||||
| Bace1 activity assay | BACE1 (human) | http:// | dnasuwww.anaspec. | orgcom/ | DNASUproducts/ | GetCloneDetailproduct. | doasp? | cloneidid= | 7823657888 |
Appendix
data sets