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Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
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| Experiment | Expected Results | Controls | Protocols | Parts | Completed? |
|---|---|---|---|---|---|
| Assay down-regulation of BACE1 by U6/miRNA | |||||
| Assay transcription factor's effectiveness in promoting expression of miRNA/BACE2 | |||||
Purchases
Experiments
Seeding 24-well Plate......Transfection......FACS
| Experiment |
|---|
Demonstrate effectiveness of miRNA in down-regulating BACE1 |
Demonstrate inducible upregulation of BACE2-YFP |
Purchases
| Item | Link | Item | Link | Purchased? | |
|---|---|---|---|---|---|
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=41166 76140 | ||||
| BACE1 (mouse) | BACE1 (human) | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236 |
Options for Directly Assaying BACE2 Expression
| Link | Cost | Comments | ||||
|---|---|---|---|---|---|---|
| Bace2 activity assay | ||||||
| https://www.anaspec.com/products/product.asp?id=57836 | $435 | Not ideal, because of expense | ||||
| Bace1 activity assay | http://www. | sigmaaldrichanaspec.com/ | catalogproducts/ | searchproduct.asp? | interface=All&term=bace2&N=0+14574647&mode=match+partialmax&focus=product&lang=en®ion=US$315-$380 | Not ideal, because of expense |
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data sets