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Date
Product
Starting Plasmid
Forward Primer
Reverse Primer

Thermocycler Variables: #cycles /

annealing temp / extension time

Gel Picture
6/23Q2-BACE2-QXpEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR30 / 60C / 30sGel Picture 6/23
Q1-eYFP-Q2pEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR
Q1-BACE2-Q2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR
Q2-eYFP-QXpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR
6/24Q2-BACE2-QXpEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR35 / 56.2C / 30sGel Picture 6/24
Q1-eYFP-Q2pEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR
Q1-BACE2-Q2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR
Q2-eYFP-QXpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR
6/25Q1-BACE2-Q2pEXPR BACE2iGEM 001-2 BACE2  N-term primerFiGEM 002-2 BACE2 N-term primerR

35 / 50.2C / 30s

 

 

 

 

 

Gel Picture 6/25
Q2-BACE2-QXpEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR
Q2-BACE2-QXpEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR
Q1-eYFP-Q2pEXPR TRE:eYFPiGEM 018 eYFP N-term primerFiGEM 019 eYFP N-term primerR
Q2-eYFP-QXpEXPR TRE:eYFPiGEM 003-2 YFP C-term primerFiGEM 004-2 YFP C-term primerR
6/26



Q2-BACE2-QX

(plus betaine)

pEXPR BACE2iGEM 016 BACE2 C-term primerFiGEM 017 BACE2 C-term primerR

30 / 55C / 1m

 

 

 

 

 





Gel Picture 6/26
 


Q1

Q2-BACE2-

Q2

QX

(plus DMSO)

pEXPR BACE2iGEM
001

Q1-

2

BACE2

 N-term primerF		iGEM 002-2 BACE2 N-term primerR 

-Q2

(plus betaine)

pEXPR BACE2

Q1-BACE2-Q2

(plus DMSO)

pEXPR BACE2
6/27Q2-BACE1-QXpEXPR BACE1iGEM 023 BACE1 C-term primerFiGEM 022 BACE1 C-term primerR Gel Picture 6/27
Q2-eYFP-QXpEXPR eYFPiGEM 012 eYFP  C-term primerFiGEM 013 eYFP C-term primerR
6/30Q1-eYFP-Q2pEXPR eYFP Gel Picture 6/30
Q1-BACE1-Q2pEXPR BACE1
7/9   Q2-eYFP-Q2QX (for BACE2)pEXPR eYFP

35 / 58C / 1m

with both DMSO and Betaine

     		  Gel Picture 7/9     
Q1-eYFP-Q2 (for BACE2)pEXPR eYFP
Q2-eYFP-QX (for BACE1)pEXPR eYFP
Q2-BACE2-QXpEXPR BACE2
Q1-BACE2-QXpEXPR BACE2
Q1-BACE1-Q2QXpEXPR BACE1
7/10(miRNA generator stuff) 

35 / 58C / 1m

with both DMSO and Betaine

Gel Picture 7/10
(20 PCR products in all) 
7/11(miRNA generator stuff)  30 / 61C / 30s Gel Picture 7/11
(12 PCR products in all) 
7/16(miRNA generator stuff)  30 / 63C / 30s

Gel Picture 7/16

 (12 PCR products in all)   

 

Ran PCRs according to protocol on NEB website: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531

...