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Safety guidelines for ALL microscopy core users.

Table of Contents

Who should read?

The microscope core is designated a BL1 space. All core users must be familiar with these general safety guidelines. Most of these are standard laboratory practices at the Koch Institute.

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Unfixed samples that include ANY human tissue or live human or primate primary cells or cell lines are considered Risk Group 2 agents and require BL2-level imaging. Imaging of live Risk Group 2 (BL2) samples is now possible, but is strictly controlled. Users wishing to image BL2 samples must read the [BL2 Safety Policies](safety-bl2.md)

Please check with core personnel if you are unsure if your sample is BL1 or BL2.

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  • No eating or drinking in the core.
  • Clothing must be appropriate lab wear. Cover your legs and wear closed shoes. No shorts or sandals!
  • Use secondary containers and at least one glove off when transporting specimens between individual microscope rooms or within the building. Use service elevators for transport.
  • Microscope decontamination guides are posted in each microscope room. Follow the decontamination procedure both after you finish. Decontaminate microscope surfaces, eyepieces, stage, keyboard, mouse, etc as directed in the posted guide. You may also opt to decontaminate before you begin.

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