Page History

** PLEASE KEEP THE FILTER CUBES IN THE BASE STATE AFTER USE **

Filter sets from Chroma http://www.chroma.com

5X Fluorescence Filter Cubes: (EX: Excitation, DM: Dichromatic Mirror, BA: barrier filter emission)

Wiki Markup*c.f.* To choose right filter combinations for fluorescent proteins \ -> \ [http://www.microscopyu.com/tutorials/java/fluorescence/fpfilterfinder/index.html\]

==========================================================================================================================================

Microscope 1

1. RTDP : oxygen dye: EX:450/50nm, DM:485nm, BA: HQ550 (longpass, LP)unmigrated-wiki-markup

*2*. FITC \ -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm *Dyes*:FITC, RSGFP, Bodipy Bodipy, Fluo 3, Dio, GFP, Alexa488 \Alexa488 [41001\]  

3. Empty

4. Empty 

5. Empty  

6. Empty

Fluorescence Illuminator : X-cite 120 from Exfo

...

==============================================================================================================================================

Microscope 2

1. Empty

Wiki Markup*2*. FITC \ -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm *Dyes*:FITC, RSGFP, Bodipy Bodipy, Fluo 3, Dio, GFP, Alexa488 \Alexa488 [41001\]  \\] 

3. GREEN Wiki Markup*3*. GREEN Chroma-> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP) * Dyes*:TRITC, Rhodamine 237, RH414, RH421, RH795, LDS751, Proqidium Iodine, RFP \RFP [11002v2-GREEN\]unmigrated-wiki-markup

*4*. Yellow GFP BP  Yellow GFP BP -> HQ500/20nm, DM:Q515nm (longpass, LP), BA: HQ535/30nm *30nm Dyes*: YFP \ [41028\]

Wiki Markup*5*. UV . UV -> EX:350/50nm (i.e. 325-375nm), DM:400nm (longpass, LP),  BA BA: 420nm (longpass, LP) * Dyes*:DAPI, Hoechst 34580, AMCA \ [11000v3-UV\]

Wiki Markup*6*. Cyan GFP \ -> D436/20nm, DM:455nm (longpass, LP), BA: D480/40nm *40nm Dyes*: CFP \ [31044v2\]

Note: The basic blue and green filter sets are designed primarily for viewing the fluorescence of a single fluorochrome. Both filter sets contain longpass emission filters which cannot exclude the fluorescence of a second fluorchrome which also may be excited. The basic green filter set is recommended for use with mercury lamps only.

Fluorescence Illuminator :unmigrated-wiki-markup

*===============================================================================================================================================* Extra filter sets :  1)
Extra filter sets :  1) HPTS-> EX:425/40nm, DM:460nm (longpass, LP), BA: D540/50 *Dyes*: \ [31007\]]

                          2) BLUE Chroma-> EX: Wiki Markup                          2) BLUE Chroma-> EX: D470/40nm, DM:495nm (longpass, LP), BA: 515nm (longpass, LP) * Dyes*: Acridine Orange (both DNA & RNA), Auramine, EGFP, FITC, S65T, RSGFP\[11001v2-BLUE\]

                          3) Cy5 -> EX: 590/60 nm, DM: 660 (longpass, LP), BA: 638/75 Dyes: Cy5

===============================================================================================================================================

Scope 1 (at MIT until December 2015)

1.  FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm     (dye: SYTO9)

2. "Green" -> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP)   (ring labeled: d546/10 x 61929)      (dye: Propidium Iodide)

3. empty

4. YFP ->  HQ500/20nm, DM:Q515nm (longpass, LP), BA: HQ535/30nm

5. "DAPI" -> EX:350/50nm (i.e. 325-375nm), DM:400nm (longpass, LP),  BA: 420nm (longpass, LP) [but labeled  550 LP] (ring labeled: d350/50 110295) 

6. "Cyan" -> D436/20nm, DM:455nm (longpass, LP), BA: D480/40nm