** PLEASE KEEP THE FILTER CUBES IN THE BASE STATE AFTER USE **
Filter sets from Chroma http://www.chroma.com
5X Fluorescence Filter Cubes: (EX: Excitation, DM: Dichromatic Mirror, BA: barrier filter emission)
c.f. To choose right filter combinations for fluorescent proteins -> [http://www.microscopyu.com/tutorials/java/fluorescence/fpfilterfinder/index.html]
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Microscope 1
1. RTDP : oxygen dye: EX:450/50nm, DM:485nm, BA: HQ550 (longpass, LP)
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4. Empty
5. Empty
6. Empty
Fluorescence Illuminator : X-cite 120 from Exfo
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Microscope 2
1. Empty
2. FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm Dyes:FITC, RSGFP, Bodipy, Fluo 3, Dio, GFP, Alexa488 [41001]
3. GREEN Chroma-> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP) Dyes:TRITC, Rhodamine 237, RH414, RH421, RH795, LDS751, Proqidium Iodine, RFP [11002v2-GREEN]
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6. Cyan GFP -> D436/20nm, DM:455nm (longpass, LP), BA: D480/40nm Dyes: CFP [31044v2]
Note: The basic blue and green filter sets are designed primarily for viewing the fluorescence of a single fluorochrome. Both filter sets contain longpass emission filters which cannot exclude the fluorescence of a second fluorchrome which also may be excited. The basic green filter set is recommended for use with mercury lamps only.
Fluorescence Illuminator :
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3) Cy5 -> EX: 590/60 nm, DM: 660 (longpass, LP), BA: 638/75 Dyes: Cy5
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Scope 1 (at MIT until December 2015)
1. FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm (dye: SYTO9)
2. "Green" -> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP) (ring labeled: d546/10 x 61929) (dye: Propidium Iodide)
3. empty
4. YFP -> HQ500/20nm, DM:Q515nm (longpass, LP), BA: HQ535/30nm
5. "DAPI" -> EX:350/50nm (i.e. 325-375nm), DM:400nm (longpass, LP), BA: 420nm (longpass, LP) [but labeled 550 LP] (ring labeled: d350/50 110295)
6. "Cyan" -> D436/20nm, DM:455nm (longpass, LP), BA: D480/40nm